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BS ISO 4831:2006

Current

Current

The latest, up-to-date edition.

Microbiology of food and animal feeding stuffs. Horizontal method for the detection and enumeration of coliforms. Most probable number technique

Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

11-30-2006

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Culture media and diluents
6 Apparatus and glassware
7 Sampling
8 Preparation of the test sample
9 Procedure
10 Calculation and expression of results
11 Precision
12 Test report
Annex A (normative) Flowcharts for procedure
Bibliography

Provides general guidelines for the detection and the enumeration of coliforms.

Committee
AW/9
DevelopmentNote
Supersedes BS 5763-3(1991) and 04/30114058 DC (12/2006)
DocumentType
Standard
Pages
18
PublisherName
British Standards Institution
Status
Current
Supersedes

This International Standard gives general guidelines for the detection and the enumeration of coliforms. It is applicable to

  • products intended for human consumption and for the feeding of animals, and

  • environmental samples in the area of food production and food handling.

Enumeration is carried out by calculation of the most probable number (MPN) after incubation in a liquid medium at 30 °C or 37 °C.

NOTE The temperature is subject to agreement between the parties concerned. In the case of milk and milk products, the temperature of incubation is 30 °C.

This enumeration method is applicable when the number sought is expected to be in the range 1 to 100 per millilitre or per gram of test sample.

A limitation on the applicability of this International Standard is imposed by the susceptibility of the method to a large degree of variability. The information given in Clause 11 provides guidance on the applicability of the method and on the interpretation of the results.

Standards Relationship
ISO 4831:2006 Identical

ISO/TS 11133-1:2009 Microbiology of food and animal feeding stuffs Guidelines on preparation and production of culture media Part 1: General guidelines on quality assurance for the preparation of culture media in the laboratory
ISO 7218:2007 Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations
ISO/TS 11133-2:2003 Microbiology of food and animal feeding stuffs Guidelines on preparation and production of culture media Part 2: Practical guidelines on performance testing of culture media
ISO 8261:2001 Milk and milk products General guidance for the preparation of test samples, initial suspensions and decimal dilutions for microbiological examination
ISO 4832:2006 Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of coliforms Colony-count technique

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