1 - AS 4276.1-1995 WATER MICROBIOLOGY - GENERAL INFORMATION AND PROCEDURES
1 - PREFACE
1 - METHOD
1 - 1 SCOPE
1 - 2 REFERENCED DOCUMENTS
2 - 3 TYPES OF WATER
2 - 4 USE OF THE METHODS
2 - 5 GOOD LABORATORY PRACTICE
3 - 6 SAMPLING AND SAMPLES
3 - 6.1 General
3 - 6.2 Storage of samples
3 - 7 GLASSWARE
3 - 8 CLEANING OF NEW GLASSWARE, RUBBERWARE, AND NON-STERILE PLASTICS
3 - 8.1 New borosilicate glass, rubberware, and non-sterile plastics
3 - 8.2 New soda glass
4 - 9 CLEANING OF USED LABORATORY EQUIPMENT
4 - 9.1 Preliminary treatment
4 - 9.2 Use of washing machines
4 - 9.3 Test tubes, Petri dishes, flasks and bottles
4 - 9.4 Pipettes
4 - 9.5 Rubber stoppers and sealing liners
4 - 9.6 Caps
5 - 10 STERILIZATION OF EQUIPMENT
5 - 10.1 General
5 - 10.2 Oven method
5 - 10.3 Autoclave method
5 - 11 MEDIA, DILUENTS AND REAGENTS
6 - 12 REFERENCE CULTURES
6 - 13 INCUBATION TEMPERATURES
6 - 14 DILUTIONS
6 - 14.1 General
6 - 14.2 Preparation of dilutions
6 - 15 GENERAL PROCEDURE FOR ESTIMATING THE MOST PROBABLE NUMBER (MPN) OF MICROORGANISMS
6 - 15.1 Principle
6 - 15.2 Diluents and culture media
6 - 15.3 Apparatus
7 - 15.4 Preparation of dilutions and inoculation of medium
7 - 15.4.1 General
7 - 15.4.2 Procedure
7 - 15.5 Incubation
7 - 15.6 Calculation
9 - 16 GENERAL PROCEDURE FOR COLONY COUNTS USING MEMBRANE FILTRATION
9 - 16.1 Principle
9 - 16.2 Diluents and culture media
9 - 16.3 Apparatus
11 - 16.4 Preparation of apparatus and materials
11 - 16.4.1 Sterilization
11 - 16.4.2 Medium
11 - 16.5 Filtration procedure
11 - 16.5.1 General
11 - 16.5.2 Procedure
12 - 16.6 Incubation
12 - 16.7 Staining, counting and identification
12 - 16.8 Calculation
13 - 17 REFERENCES